Thesis writing Botany: TEST SYSTEM & TREATMENT SCHEDULE

TEST SYSTEM & TREATMENT SCHEDULE: Allium cepa –Taken as a model plant, Bulbs were germinated in sterilized moist sand, Seedlings then transferred to the hydroponic medium. PREPARATION OF GROWING MEDIUM · Half strength modified Hoagland Medium selected for hydroponics · Control - Normal Hoagland Medium · Experiment 1- Iron Chelate in nutrient medium replaced with Iron oxide Nanoparticle · Experiment II - Copper sulfate in nutrient medium replaced with Copper oxide Nanoparticle Various concentrations (mM) of Iron Oxide and Copper Oxide NPs in suspension form prepared in deionized water- NPs suspended in deionized water-Dispersed using magnetic stirrer-Ultrasonicated for 30 min. Approved objectives of research topic: · To investigate the impact of metal oxide NPs in the vegetative system. · To analyze phytotoxicity of NPs for cytogenetic abnormalities in the roots. · To determine the penetration and translocation of NPs in organelles and tissues by TEM images of vegetative parts. · To determine the uptake of elements in the xylem sap and control by ICP-MS and ICP-AES. · To determine the oxidative damage to [login to view URL] tissues after intake of nanoformulation · To find the disease resistance potential of NP exposed [login to view URL] inoculated with Fusarium oxysporum.